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Objective Titanium alloy productions’ surface characteristics (white and black) of two kinds of different processing technology are identified，and hemocompatibility is evaluated to provide theoretical basis for choosing suitable technological process.The surface composition and hemocompatibility of titanium alloys (white and black) using two kinds of different polishing techniques are identified and evaluated.Methods The structure and surface physical and chemical properties of the titanium alloys were characterized by scanning electron microscopy (SEM)，energy dispersive spectroscopy (EDS)，atomic force microscopy (AFM)，X-ray diffraction (XRD)，X-ray photoelectron spectrometer (XPS). Hemocompatibility of titanium alloys was systematic evaluated by in vitro fibrinogen adsorption，platelet adhesion，platelet activation，clotting time，and in vivo implantation into dog’s right atrium for 10days.Results The surface of white titanium alloy was thick and deep furrows and the ingredients were Ti，Al，V.The surface of black titanium alloy was layered and superposed distribution，and the compositions were Ti，Al，V and some impurities such as Si，Na，Ca，Fe，etc.The results of XRD showed that the phase structures of the two titanium alloys were titanium.XPS results showed that the surfaces of both the titanium alloys form a very thin TiO2 film，and the thin film of black titanium alloy was slightly thicker than white titanium alloy.The results of the two kinds of titanium alloys’ hemocompatibility had no significant difference in the experiments of fibrinogen adsorption，platelet adhesion，platelet activation，clotting time in vitro.In vivo implantation experiment，the results showed that the surfaces of both kinds of titanium alloys formed thrombus.Black titanium alloy possessed markedly foreign body reaction and formed hyperplastic granulation tissue.And the visible components of surface adhesion in white titanium alloy were less than black titanium alloy.Conclusions Combined with in vivo and in vitro experiments，the biocompatibility of white titanium alloy is superior to the black titanium alloy.