北京生物醫(yī)學(xué)工程

三維細(xì)胞培養(yǎng)中支架的研究現(xiàn)狀

Research advance in scaffords of three-dimensional cell culture

作者：李彤張曉曉李婧王俊妹吳哲

單位：吉林大學(xué)口腔醫(yī)學(xué)院(長春130021) 

關(guān)鍵詞： 3D細(xì)胞培養(yǎng)；生理微環(huán)境；支架；細(xì)胞外基質(zhì)

分類號(hào)：R318;Q813.1

出版年·卷·期（頁碼）：2017·36·4（433-437）

摘要：

細(xì)胞培養(yǎng)技術(shù)是生物技術(shù)中最核心、最基礎(chǔ)的技術(shù)，目前常用的二維細(xì)胞培養(yǎng)方法,例如，多孔板、燒瓶和培養(yǎng)皿等存在諸多不足和限制，三維細(xì)胞培養(yǎng)能夠改善細(xì)胞局部微環(huán)境、促進(jìn)細(xì)胞與細(xì)胞、細(xì)胞與基質(zhì)之間的交流，模擬體內(nèi)生理環(huán)境，從而增強(qiáng)細(xì)胞功能，在新藥研發(fā)、腫瘤研究等領(lǐng)域具有廣泛應(yīng)用前景。支架是實(shí)現(xiàn)三維細(xì)胞培養(yǎng)最常用的手段之一，相當(dāng)于人工細(xì)胞外基質(zhì)，能夠模擬體內(nèi)組織復(fù)雜的三維結(jié)構(gòu)及其主要特征。本文從基于支架的培養(yǎng)體系和無支架的培養(yǎng)體系兩方面討論了三維細(xì)胞培養(yǎng)方法，尤其詳細(xì)介紹了各種支架。

Cell culture technology is one of the most core and basic technologies in biotechnology.At present,the limitation and shortcomings of two-dimensional cell culture methods such as multi-well plates,flasks and Petri dishes are obvious.Three-dimensional cell culture can improve the local microenvironment,cell-cell and cell-extracellular matrix interactions,mimic the physiological conditions in vivo to enhance the cell function.Three-dimensional cell culture might be widely used in oncology,drug discovery and other fields in the future.Scaffold is one of the most popular methods for three-dimensional cell culture,which is equivalent to artificial extracellular matrix to mimic the complex three-dimensional structure and the main characteristics of living tissues.This review summarizes three-dimensional cell culture from two aspects:scaffold-based and scaffold-free three-dimensional cell culture,especially introduces the scaffolds.

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