北京生物醫(yī)學工程

高血壓大鼠視網膜血瘀出血圖像采集與處理

Image acquisition and processing of retinal bloodstasis in hypertensive rats

作者：張魁星張方申彭欣

單位：山東中醫(yī)藥大學理工學院(濟南250355)

分類號：R318.04

出版年·卷·期（頁碼）：2017·36·5（441-445）

摘要：

目的研究高血壓大鼠視網膜眼底圖像的采集與分析方法，以應用于血瘀出血機制研究。方法實驗分2組，對照組為20只健康雄性Wistar大鼠，模型組為20只雄性高血壓大鼠(SBP≥140mmHg)，利用視網膜照相機及微循環(huán)顯微鏡采集視網膜圖像；運用圖像處理技術計算動靜脈管徑比(DA/DV)、出血面積等形態(tài)學參數等。結果模型組大鼠視網膜微血管數量減少,DA/DV 明顯減小,血液流速減慢,與對照組相比，差異均具有統(tǒng)計學意義(P<0.05)。高血壓大鼠視網膜圖像存在著明顯的瘀血出血現象；經圖像處理后，此病理變化的圖像特征可以得到清晰的量化表達。結論從微血管形態(tài)學角度，明確了高血壓模型存在著血瘀出血的病理改變；同時，也為高血壓血管損害與治療效果的評判，提供了深入研究的有效方法及客觀評價標準。

Objective To study the preparation and quantitative evaluation criteria of blood stasis image of hypertensive rats. Methods The rats were divided into the model group (20 male hypertensive rats, SBP≥140mmHg) and the control group (20 male healthy Wistar rats).Retinal camera and mesenteric microcirculation observation system were used to perform the observation.The image processing technique was used to calculate the morphological parameters,such as the diameter ratio of artery to vein and the area of bleeding. Results The number of the retinal microvascular in the model groupdecreased,DA/DV decreased, blood flow reduced, compared with the control group,the differences were statistically significant (P<0.05). Retinal images of hypertensive rats had obvious blood stasis and bleeding phenomenon; after processing,image characteristics of the pathological changes could get a clear quantitative expression. Conclusions From the perspective of microvascular morphology,there is a clear pathological changes of blood stasis model of hypertension hemorrhage. At the same time, for blood vessels and therapeutic effect evaluation,it also provides some effective methods for further research and objective evaluation criteria.

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