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目的 提出一種新型的微滴生成方式——油包水(W/O)包裹技術(shù)，為數(shù)字PCR實驗過程中微滴的制備提供一種更便捷的方式。方法 通過配制一定比例的特定油相以及包含模板DNA等反應物的特定水相，在不同條件下形成W/O微滴，并進行PCR反應。結(jié)果 所形成的微滴粒徑大小均勻，并且在進行PCR反應過程中熱力學性能保持不變，微滴不會因溫度升降而破裂，適合于數(shù)字PCR中微滴的制備。結(jié)論 生成的微滴可以有效地使模板DNA進行擴增反應。

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