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Objective In order to determine the latent ischemic myocardium in a more comprehensive way and assess cardiopulmonary function and diagnosis-related diseases, this paper develope a new exercise ECG measurement system, and preliminary verify the reliability of its clinical applications. Methods Firstly, the system combined respiratory mechanics monitoring, carbon dioxide monitoring, and oxygen monitoring with traditional exercise ECG systems. Secondly, we adopted a new QRS onset and offset detection algorithm, adaptive filtering and other techniques to avoid the effect of motion noise on ECG and blood pressure values.Finally, the standard compliance test and clinical test were conducted to prove the performance of the new system. Results The system passed the standard compliance test, which proved that the key technical indicators such as common mode rejection ratio and input noise of the system meet the standard requirements. Whatis more, the clinical test showed that the standard deviation of HR, ST, ST/HR, SP/DP between gauged by the new exercise ECG measurement system and physicians were 0. 798 beat/min, 0. 008 8 m V, 0. 071 u V/ (beat/min) , 1. 135/0. 919 mm Hg (1 mm Hg = 133. 322 Pa) . Conclusions Thenew exercise ECG measurement system proposed in this paper is stable and reliable, and the ECG, blood pressure and other parameters could be calculated accurately, which provides a reliable reference for cardiopulmonary function assessment and has high clinical application value.