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Objective Magnesium silicate hollow spheres ( MgSiO3, denoted as NPs) are getting more attentions in biomedical applications due to its high surface area and mesoporous structures. Therefore, to study potential applications of NPs as protein drug delivery, we synthesize NPs by template method and analyze their cytocompatibility, degradation, adsorption and release properties of proteins. Methods We used nano SiO2 as template to synthesis NPs, and TEM, SEM, DLS and XRD techniques were applied to characterize the morphologies and structures of obtained NPs. CCK-8 experiments were used to test cytotoxicity of synthesized NPs. Moreover, we studied the protein absorption and release of NPs by UV-Vis spectrophotometry, and Langmuir, Freundlich and Temkin isothermal adsorptionmodels were applied to analysis the adsorption behavior. Results Characterization of synthesized NPs exhibited uniform hollow spheres and high surface area ( 412. 35 m2/g) . In cell experiments, cell viability were about90% even at high concentration of NPs ( 250 μg/m L) . In degradation experiments, synthesized NPs degraded80% in acid environments. In protein adsorption and release experiments, when the protein concentration was3. 2 mg/m L, the loading amount of protein could be 225 mg/g. In addition, loading protein could be released in neutral and acidic conditions, especially in acidic conditions. In adsorption isotherm model experiments, Freundlich adsorption isotherm model fitted well with BSA adsorption data. Conclusions Therefore, the synthesized MgSiO3 hollow spheres exhibit good biocompatibility and degradation property, high protein loading capacity, and release of protein as pH-controllable. So, these NPs are more promising for applications in biomedical fields, for example, protein adsorption and delivery.