北京生物醫(yī)學(xué)工程

基于超聲壓痕技術(shù)的兔眼角膜生物力學(xué)特性

Biomechanical properties of rabbit cornea based on ultrasonic indentation

作者：田磊韓為秦曉李林王立科張海霞

單位：首都醫(yī)科大學(xué)附屬北京同仁醫(yī)院北京同仁眼科中心北京市眼科研究所；眼科學(xué)與視覺科學(xué)北京市重點(diǎn)實(shí)驗(yàn)室(北京 100730) 首都醫(yī)科大學(xué)生物醫(yī)學(xué)工程學(xué)院(北京 100067) 首都醫(yī)科大學(xué)臨床生物力學(xué)應(yīng)用基礎(chǔ)研究北京市重點(diǎn)實(shí)驗(yàn)室(北京 100069) 香港理工大學(xué)生物醫(yī)學(xué)工程跨領(lǐng)域?qū)W部(香港 999077)

關(guān)鍵詞：超聲壓痕技術(shù)；彈性模量；眼內(nèi)壓；眼角膜；生物力學(xué)

分類號(hào)：R318.01

出版年·卷·期（頁碼）：2019·38·2（159-165）

摘要：

目的利用超聲壓痕技術(shù)探索兔眼角膜的生物力學(xué)特性。方法選取7月齡新西蘭白兔眼球7只，制作完整角膜試樣并固定于人工前房上。用微量注射泵給人工前房注水改變前房內(nèi)的壓力,并用壓力傳感器測量；在不同前房壓力狀態(tài)下，利用超聲壓痕設(shè)備在角膜頂點(diǎn)位置進(jìn)行壓痕實(shí)驗(yàn)，獲得力-位移曲線。研究角膜力學(xué)參數(shù)（彈性模量、滯回量等）與前房壓力的關(guān)系。結(jié)果由角膜壓痕實(shí)驗(yàn)所得的力-位移曲線呈現(xiàn)非線性特點(diǎn)。前房內(nèi)壓力越大，角膜的力-位移曲線越陡峭。兔眼角膜的彈性模量隨前房壓力升高而增大，在前房壓力為7~45 mmHg范圍內(nèi)，其數(shù)值范圍為0.30~1.55 MPa。隨前房壓力增大，角膜滯回量呈現(xiàn)線性上升趨勢(shì)。結(jié)論基于超聲壓痕技術(shù)獲得的兔眼角膜彈性模量隨前房壓力呈線性增大，滯回量等角膜黏性特性參數(shù)與前房壓力也相關(guān)。

Objective The biomechanical properties of rabbit corneas in vitro were explored by ultrasonic indentation experiment. Metheds Seven eye balls of New Zealand white rabbit with 7-month-old were used and the corneas were fixed on an artificial anterior chamber. The pressure in the anterior chamber were changed by injecting or ejecting normal saline in the artificial anterior chamber by a microinjection pump, and the intraocular pressure in the chamber was measured in real time by a pressure sensor. Indentation experiment was performed on the corneal apex by the ultrasonic indentation device at different pressure states, and the relationships between corneal biomechanical parameters and intraocular pressure were studied. Results The force-displacement curves of cornea in loading and unloading process were nonlinear. The higher the intraocular pressure, the steeper the load-displacement curves. At the intraocular pressure range of 7 to 45 mmHg, corneal tangent modulus increased with pressure with the value ranging between 0.30 to 1.55 MPa. The amount of hysteresis indicated the energy consumption of the cornea during loading and unloading process, was related to the viscous of the cornea. The result showed that the greater the pressure, the greater the amount of hysteresis would be. Conclusions Ultrasonic indentation experiments show that the corneal tangent modulus increases linearly with intraocular pressure and the viscous parameters are related with intraocular pressure.

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