北京生物醫(yī)學(xué)工程

一種高通量測(cè)量單細(xì)胞彈性模量的微流控芯片

A high-throughput microfluidic chip for trapping single cells and measuring single cells’ elastic moduli

作者：袁闈墨薛春東劉波覃開蓉

單位：大連理工大學(xué)電子信息與電氣工程學(xué)部生物醫(yī)學(xué)工程學(xué)院( 遼寧大連 116024) 大連理工大學(xué)光電工程與儀器科學(xué)學(xué)院( 遼寧大連 116024)

關(guān)鍵詞：微流控; 芯片實(shí)驗(yàn)室; 有限元分析; 生物力學(xué)

分類號(hào)： R318.6

出版年·卷·期（頁碼）：2019·38·5（450-456）

摘要：

目的設(shè)計(jì)微流控芯片以便高效簡(jiǎn)便地捕獲大量單細(xì)胞并測(cè)量其彈性模量。方法根據(jù)流體力學(xué)原理，設(shè)計(jì)微流控陣列及其單細(xì)胞捕獲單元的通道結(jié)構(gòu)和幾何尺寸。培養(yǎng)海拉細(xì)胞，制作微流控芯片實(shí)物并采用該芯片進(jìn)行單細(xì)胞捕獲實(shí)驗(yàn)。采用COMSOL軟件對(duì)作用在被捕獲細(xì)胞上的剪切力和壓差進(jìn)行有限元仿真。根據(jù)作用在被捕獲細(xì)胞兩側(cè)的壓差值和細(xì)胞在捕獲通道中的伸長(zhǎng)長(zhǎng)度，計(jì)算出細(xì)胞的彈性模量。結(jié)果所設(shè)計(jì)的微流控芯片能有效捕獲大量單細(xì)胞；計(jì)算的單細(xì)胞彈性模量為780.7Pa ± 100.5 Pa，與文獻(xiàn)中報(bào)道的763Pa ± 93 Pa接近。結(jié)論本文所提出的微流控芯片可高效捕獲單細(xì)胞并測(cè)量單細(xì)胞力學(xué)特性。

Objective To design a microfluidic chip to efficiently and conveniently trap substantial single cells and measure single cells’ elastic moduli. Method Based on the principle of fluid mechanics, a microfluidic array and the structure and geometrical size of its single cell trapping unit were designed. Hela cells were cultured and maintained, the actual microfluidic chip was then fabricated to conduct the single cells trapping experiment. By using software COMSOL, finite element simulation was conducted to compute the shear stress and pressure drop across the trapped cells. The single cells’ elastic moduli were computed by the pressure drops across the trapped single cells and the cellular protrusion lengths in the trapping channels. Results Many single cells were able to be efficiently trapped in the microfluidic chip. The computed elastic moduli of single cells were 780.7 Pa± 100.5 Pa, which were close to the moduli 763Pa ± 93 Pa reported in the literature. Conclusions The microfluidic chip proposed in this study exhibites a high efficiency for single cells trapping and measurement of single cellular mechanical property.

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