北京生物醫(yī)學(xué)工程

兔眼虹膜形態(tài)隨眼內(nèi)壓變化的體內(nèi)實(shí)驗(yàn)研究

Experimental research on the morphological changes of rabbit irisin vivowith the rise of intraocular pressure

作者：張昆亞錢秀清吳寶平王輝劉志成

單位：首都醫(yī)學(xué)大學(xué)生物醫(yī)學(xué)工程學(xué)院（北京 100069）；首都醫(yī)科大學(xué)臨床生物力學(xué)應(yīng)用基礎(chǔ)研究北京市重點(diǎn)實(shí)驗(yàn)室（北京 100069）

關(guān)鍵詞：虹膜；形態(tài)參數(shù)；高眼壓；玻璃體灌注；青光眼

分類號(hào)：R318

出版年·卷·期（頁碼）：2019·38·6（605-610）

摘要：

目的獲得動(dòng)物急性高眼壓下的虹膜形態(tài)變化，為深入認(rèn)識(shí)伴虹膜形態(tài)變化的青光眼病程發(fā)展提供幫助。方法選用新西蘭白兔7只，基于自主搭建的實(shí)驗(yàn)平臺(tái)，對(duì)兔眼玻璃體進(jìn)行穿刺，快速灌注生理鹽水。灌注過程中，通過傳感器記錄前房眼壓變化，通過小動(dòng)物超聲影像裝置記錄虹膜的形變圖像。為了量化虹膜的形態(tài)變化，選取虹膜面積、長度、上弧長和下弧長作為虹膜的形態(tài)參數(shù)。通過圖像和數(shù)據(jù)處理，獲得虹膜的形態(tài)參數(shù)隨眼內(nèi)壓的變化規(guī)律。結(jié)果玻璃體灌注過程中，眼內(nèi)壓升高，晶體前移，虹膜內(nèi)緣前移。虹膜各形態(tài)參數(shù)隨眼內(nèi)壓的升高均呈現(xiàn)下降趨勢，二者關(guān)系可以通過公式

s = b₀+ b₁lnp 進(jìn)行擬合，其中，s為任一形態(tài)參數(shù)，p為眼內(nèi)壓，b₀和b₁均為擬合參數(shù)。結(jié)論玻璃體灌注可以快速升高眼內(nèi)壓，并伴隨虹膜的形態(tài)變化。本研究為深入了解伴虹膜形態(tài)變化的青光眼病程提供了實(shí)驗(yàn)基礎(chǔ)。

Objective Our purpose is to qualify morphological changes of the iris in the acute ocular hypertension animal model, which may be helpful to know the process of angle closure glaucoma with the morphological changes of the iris. Methods Experiments are conducted on seven New Zealand white rabbits based on the self-built experimental platform. Normal saline is infused to the vitreous body. During the infusion, the sensor is used to monitor the IOP (intraocular pressure), and morphology of the iris is captured by the small animal ultrasonic imaging system. In order to quantify the morphological changes of the iris well, certain morphometric parameters are chosen such as cross-sectional area, length, upper arc length and lower arc length. Moreover, the relationships between the morphometric parameters and the IOP are obtained by image processing and data processing. Results Normal saline infusion to the vitreous body increases the IOP. With the rise of the IOP, both the lens and pupillary margin of the iris move forward. Meanwhile, the iris morphometric parameters change. Furthermore, the fitting formula between the morphological parameters and the IOP are established, which can be described as s = b₀+ b₁lnp while s is any morphometric parameter, p is IOP, b₀ and b₁ are both fitting parameters. Conclusions The vitreous body infusion could induce acute ocular hypertension with morphological changes of the iris, which gives insight to the process of glaucoma with the morphological changes of the iris.

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