北京生物醫(yī)學(xué)工程

胍基抗菌材料在糖尿病動物皮膚創(chuàng)傷中的抗菌性能研究

Antibacterial properties of guanidine materials on wounds of diabetic animal skin

作者：邱浩峰馮培培駱陽吳徐金侯文佳牟賢波竺亞斌

單位：寧波大學(xué)醫(yī)學(xué)院（浙江寧波 315211）

關(guān)鍵詞：聚六亞甲基胍；胍基聚合物; 糖尿病; 皮膚創(chuàng)傷; 抗菌

分類號：R318.08

出版年·卷·期（頁碼）：2020·39·3（233-243）

摘要：

目的糖尿病可以引起多種并發(fā)癥，皮膚潰瘍、潰爛是常見癥狀，并且經(jīng)久不愈。因此，本研究擬制備一種性能優(yōu)異的胍基抗菌材料，以對糖尿病患者皮膚潰瘍、潰爛的治療提供參考。方法首先將鹽酸胍與己二胺發(fā)生聚合反應(yīng)，合成具有良好抗菌性能的系列聚六亞甲基胍（polyhexamethylene guanidine，PHMG），并用紅外光譜分析檢測PHMG的分子結(jié)構(gòu)；然后對其進(jìn)行抗菌性能檢測，菌種包括大腸桿菌、耐甲氧西林金黃色葡萄球菌MRSA USA300、銅綠假單胞菌PAO1和PGO2330；最后，構(gòu)建糖尿病模型小鼠，并造成小鼠皮膚損傷，在未染菌和感染金黃色葡萄球菌（MRSA USA300）兩種情況下，對PHMG的殺菌和抗感染性能進(jìn)行研究。結(jié)果聚合反應(yīng)發(fā)生，成功合成系列PHMG。其中，PHMG5對上述4種細(xì)菌具有很好的抗菌效果。動物實(shí)驗(yàn)證明，PHMG5對于糖尿病小鼠皮膚傷口具有顯著抗菌性能。結(jié)論通過聚合反應(yīng)可以合成系列聚六亞甲基胍，該胍基聚合物具有良好的抗菌性能，有利于糖尿病小鼠傷口的抗感染。

Objective Severe diabetic patients are always accompanied by refractory skin ulceration, since the patients possessed abnormal insulin regulation and weak immunity which leaded to the poor resistance to foreign microorganism infection. Therefore, the purpose of this study is to synthesize a guanidine-based material with excellent antibacterial properties, which will be valuable for the treatment of diabetic skin ulcers in future. Methods Polyhexamethylene guanidines (PHMGs) with good antibacterial properties were synthesized based on the polymerization reaction of guanidine diamine with hexamethylenediamine under appropriate conditions. The molecular structure of PHMG was detected by infrared spectrum analysis. Subsequently, the antibacterial properties of these materials to common bacteria were tested. The strains include: Escherichia coli(E. coli), methicillin-resistant Staphylococcus aureus (MRSA USA300), Pseudomonas aeruginosa (PAO1 and PGO2330). On this basis, diabetic mice were constructed to simulate the skin damage of clinically diabetic patients. The bactericidal and anti-infection properties of PHMGs were investigated via S. aureus MRSA USA300 diabetic wound infection model in mice. Results Infrared spectroscopy confirmed the occurrence of the polymerization reaction. The minimum bacteriostatic concentration and bacteriostatic zone experiments showed that PHMGs had good bacteriostatic effects on the above four kinds bacteria. The in vivo experiment verified that PHMGs had significant inhibitory effects on skin wounds of diabetic mice. Conclusions A series of polyhexamethylene guanidine were synthesized through polymerization between guanidine diamine and hexamethylenediamine. The guanidine-based polymer has good bacteriostatic properties and is beneficial to the anti-infection of wounds in diabetic mice.

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