北京生物醫(yī)學(xué)工程

PCL / ZrO2 骨組織工程支架 3D 打印制備方法及其性能研究

Preparation and properties of PCL / ZrO2 bone tissue engineering scaffold based on 3D printing

作者：王啟帆馬志勇鐘林娜謝雯佳錢正史耕田

單位：寧波大學(xué)機(jī)械工程與力學(xué)學(xué)院(浙江寧波 315211) 湖州師范學(xué)院工學(xué)院(浙江湖州 313000) 湖州艾先特電子科技有限公司(浙江湖州 313000) 四川大學(xué)華西口腔醫(yī)院修復(fù)科(成都 610000) 通信作者:馬志勇? E-mail:02641@ zjhu.edu.cn

關(guān)鍵詞： 3D 打印; 骨組織工程支架; 聚己內(nèi)酯; 氧化鋯; 生物相容性

分類號(hào)：R318

出版年·卷·期（頁碼）：2020·39·4（418-424）

摘要：

目的為制備出滿足骨缺損修復(fù)需要的具有一定力學(xué)強(qiáng)度和生物活性的骨組織工程支架,本文選取聚己內(nèi)酯(polycaprolactone, PCL)和納米氧化鋯( ZrO2 ) 粉末制備出三維多孔復(fù)合材料支架。方法采用高溫熔融擠出 3D 打印方式制備 PCL / ZrO2 復(fù)合材料支架,為獲取支架的幾何形態(tài)?力學(xué)性能和生物學(xué)性能,利用掃描電子顯微鏡( scanning electron microscope, SEM) 和萬能試驗(yàn)機(jī)( material testsystem,MTS)分別分析了支架的形貌和壓縮性能,并通過體外細(xì)胞培養(yǎng)的方式測(cè)試復(fù)合材料支架的生物相容性。結(jié)果制備完成的復(fù)合材料支架具有良好的三維孔隙結(jié)構(gòu),孔徑≥ 400 μm,孔隙率≥ 40 %? 對(duì)比純 PCL 支架,PCL / ZrO2 復(fù)合材料支架的力學(xué)性能顯著提高,楊氏模量提高 0倍左右,抗壓強(qiáng)度提高0倍左右。在體外實(shí)驗(yàn)中,細(xì)胞培養(yǎng) 7 d 后 PCL / ZrO2 復(fù)合材料支架上的細(xì)胞增殖對(duì)比純 PCL 支架有顯著提高。結(jié)論基于該結(jié)果,本文制備出的 PCL / ZrO2 生物活性骨組織支架在骨組織工程方面有一定的應(yīng)用前景。

【Abstract】 Objective In order to prepare bone tissue engineering scaffolds with certain mechanical strength and biological activity to meet the needs of bone defect repair, three-dimensional porous composite scaffolds were prepared by using polycaprolactone (PCL)and nano-zirconia ( ZrO2 ) powder. Methods The PCL /ZrO2 composite material support was prepared by high temperature melt extrusion 3D printing. The geometry, mechanical properties and biological properties of the scaffolds were obtained. The morphology and compression properties of the scaffolds were analyzed by scanning electron microscope (SEM) and material test system (MTS). The biocompatibility of the composite scaffold was tested by in vitro cell culture. Results The prepared composite scaffold had a good 3D pore structure, a pore diameter of ≥ 400 μm, and a porosity of ≥ 40%. Compared with the pure PCL stent, the mechanical properties of the PCL / ZrO2 composite stent were significantly improved, the Young’s modulus was increased by about 0.4 times, and the compressive strength was increased by about 0.5 times. In vitro, cell proliferation on the PCL /ZrO2 composite scaffold was significantly improved after 7 days of cell culture compared to the pure PCL scaffold. Conclusions Based on the results, the PCL / ZrO2 bioactive bone tissue scaffold prepared in this paper has certain application prospects in bone tissue engineering.

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