北京生物醫(yī)學(xué)工程

基于原子力顯微鏡壓痕技術(shù)的大鼠小梁網(wǎng)組織彈性模量測(cè)定

Determination of elastic modulus of rat trabecular meshwork by atomic force microscope indentation technique

作者：王川李林劉志成

單位：1首都醫(yī)科大學(xué)燕京醫(yī)學(xué)院（北京101300） 2首都醫(yī)科大學(xué)生物醫(yī)學(xué)工程學(xué)院（北京 100069） 3臨床生物力學(xué)應(yīng)用基礎(chǔ)研究北京市重點(diǎn)實(shí)驗(yàn)室（北京 100069） 通信作者：劉志成，教授，博士研究生導(dǎo)師。E-mail：[email protected]

關(guān)鍵詞：小梁網(wǎng)組織；原子力顯微鏡；彈性模量，壓痕技術(shù)；Hertz模型

分類(lèi)號(hào)：R318.01

出版年·卷·期（頁(yè)碼）：2021·40·4（354-359）

摘要：

目的探索基于原子力顯微鏡壓痕技術(shù)確定大鼠小梁網(wǎng)組織彈性模量的方法，為揭示小梁網(wǎng)組織力學(xué)特性與小梁網(wǎng)通道房水外流阻力之間的關(guān)系等研究奠定基礎(chǔ)。方法首先利用大鼠眼球組織切片獲取大鼠小梁網(wǎng)組織與毗鄰組織之間的距離信息和結(jié)構(gòu)信息；其次利用AFM壓痕實(shí)驗(yàn)獲取角膜-角鞏膜緣-小梁網(wǎng)-鞏膜方向的彈性模量與相對(duì)位置的關(guān)系；綜合對(duì)比以確定小梁網(wǎng)組織測(cè)試區(qū)域。結(jié)果探索了一種基于原子力顯微鏡壓痕實(shí)驗(yàn)定位小梁網(wǎng)組織并獲取該組織彈性模量的方法，利用該方法獲取了大鼠眼球鼻側(cè)、顳側(cè)、上側(cè)、下側(cè)4個(gè)位置處的小梁網(wǎng)組織彈性模量范圍為300～600 Pa。結(jié)論本研究給出了一種基于AFM壓痕技術(shù)確定大鼠小梁網(wǎng)組織彈性模量的方法，該方法提供了AFM測(cè)試環(huán)境下小梁網(wǎng)組織區(qū)域的位置參考，優(yōu)勢(shì)在于可快速定位到大鼠小梁網(wǎng)組織區(qū)域。

Objective To explore the method of determining the elastic modulus of rat trabecular meshwork（TM） based on atomic force microscope (AFM) indentation technique, and lay the foundation for revealing the relationship between the mechanical properties of TM and the outflow resistance. Methods Firstly, obtain the distance and structure information of TM and adjacent tissues by using the rat eye tissue section; secondly, the relationship between elastic modulus and relative position of cornea-corneoscleral limbus-TM-sclera was obtained by AFM indentation; the test area of TM was determined by comprehensive comparison. Results Explored a method for locating TM and obtaining its elastic modulus based on AFM indentation experiment. The elastic modulus of TM at nasal, temporal, superior and inferior sides of the eyeball was obtained by this method, which ranged from 300Pa to 600Pa. Conclusions This study provides a method to determine the elastic modulus of rat TM based on AFM indentation. This method can provide the location reference of TM area in AFM test environment, and the advantage is that it can quickly locate the TM tissue area in rats.

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