北京生物醫(yī)學(xué)工程

生物瓣抗鈣化檢測方法的研究

Study on anti-calcification detection method of biological valve

作者：于秋航段蘇然楊柳姜愛莉付海洋王召旭

單位：煙臺大學(xué)（山東煙臺264005 中國食品藥品檢定研究院（北京102629）濟寧市食品藥品檢驗檢測中心（山東濟寧 272100） 通訊作者：1.付海洋。E-mail:[email protected]    2.姜愛莉。E-mail:[email protected]

關(guān)鍵詞：生物材料；鈣；火焰-原子吸收分光光度法；電感耦合等離子體發(fā)射光譜法

分類號：R318.04

出版年·卷·期（頁碼）：2022·41·1（49-53）

摘要：

目的對火焰原子吸收分光光度法（flame atomic absorption spectrophotometry, FAAS）與電感耦合等離子體原子發(fā)射光譜法（inductively coupled plasma atomic emission spectrometry，ICP-OES）進(jìn)行方法學(xué)驗證研究，以探究兩種方法應(yīng)用于檢測生物材料鈣含量的可行性，為制定生物瓣抗鈣化性能檢測方法標(biāo)準(zhǔn)提供參考。方法將生物瓣植入3周剛斷乳雄性Wistar大鼠背部皮下，8周后取出植入物，用FAAS法和ICP-OES法檢測植入物中鈣含量，并研究兩種方法的線性、專屬性、重復(fù)性、精密度、準(zhǔn)確度及樣品檢測結(jié)果的差異。結(jié)果當(dāng)生物瓣的鈣質(zhì)量濃度為0~5.0 μg/mL時兩種方法的線性關(guān)系均良好，相關(guān)系數(shù)大于0.999。ICP-OES法測定時定量限更低、測準(zhǔn)確性更好，與FAAS法相比更為優(yōu)秀，且兩種方法的回收率與相對標(biāo)準(zhǔn)偏差都能滿足對生物材料中鈣含量測定的要求。結(jié)論 FAAS法與ICP-OES法均可作為標(biāo)準(zhǔn)方法用于生物瓣中鈣含量的測定，F(xiàn)AAS需要加入掩蔽劑，但測定過程靈活，可通過預(yù)估將檢驗液中鈣濃度稀釋至標(biāo)曲濃度范圍內(nèi)后測定；ICP-OES受干擾少，但不能預(yù)估檢驗液的鈣含量，因此適用于鈣含量范圍一定的大量樣品的檢測工作。

Objective The two methods of flame atomic absorption spectrophotometry （FAAS） and inductively coupled plasma atomic emission spectrometry （ICP-OES）are verified and compared to explore the feasibility of applying the two methods to detect the calcium content of biomaterials, so as to provide reference for formulating the detection method standard of anti calcification performance of biological valve. Methods The biological valve is implanted subcutaneously into the back of three week weaned male Wistar rats, after eight weeks, the implants are removed, the calcium content in the implants is detected by FAAS and ICP-OES, and the linearity, specificity, repeatability, precision, accuracy and sample detection results of the two methods are compared. Results When the calcium concentration of biological valve is 0 ~ 5.0 μg / mL, the linear relationship between the two methods is good, and the correlation coefficient is greater than 0.999. Compared with FAAS method, ICP-OES Method has lower quantitative limit and better accuracy, and the recoveries and relative standard deviations of the two methods can meet the requirements for the determination of calcium content in biomaterials. Conclusions Both FAAS and ICP-OES can be used as standard methods for the determination of calcium in biological valve. FAAS needs to add masking agent, but the determination process is flexible. The calcium concentration in the solution to be measured can be diluted to the standard curve concentration by estimation; ICP-OES is less disturbed, but it can not predict the calcium content of the test solution, so it is suitable for the detection of a large number of samples with a certain range of calcium content.

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