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伊班膦酸鈉聯(lián)合骨髓間充質(zhì)干細(xì)胞干預(yù)治療骨質(zhì)疏松動(dòng)物的骨組織生物力學(xué)性能研究 Study on the biomechanical properties of bone tissue of osteoporosis animals treated with ibandronate combined with bone marrow mesenchymal stem cells |
| 作者： 劉曉輝 秦璇 杜靜 郝明 高志達(dá) 潘鑠 胡亞寧 田智廣 |
| 單位：石家莊市人民醫(yī)院 骨一科（石家莊市 050000） <p>通訊作者：劉曉輝。E-mail:[email protected]</p> <p> </p> |
| 關(guān)鍵詞： 骨質(zhì)疏松；動(dòng)物模型；骨髓間充質(zhì)細(xì)胞；骨密度；生物力學(xué) |
| 分類號(hào)：R318.01 |
| 出版年·卷·期（頁碼）：2022·41·3（280-285） |
| 摘要： |
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目的 驗(yàn)證骨髓間充質(zhì)干細(xì)胞(bone mesenchymal stem cells ，BMSCs)聯(lián)合伊班膦酸鈉干預(yù)治療骨質(zhì)疏松動(dòng)物后骨的壓縮、彎曲等力學(xué)性能恢復(fù)的效果。方法 取5月齡雌性SD大鼠60只，隨機(jī)分為正常對(duì)照組12只，骨質(zhì)疏松動(dòng)物模型組12只，骨質(zhì)疏松動(dòng)物模型以伊班膦酸鈉干預(yù)組12只，骨質(zhì)疏松動(dòng)物模型以骨髓間充質(zhì)干細(xì)胞干預(yù)組12只，骨質(zhì)疏松動(dòng)物模型以伊班膦酸鈉聯(lián)合骨髓間充質(zhì)干細(xì)胞干預(yù)組12只。以摘除大鼠卵巢的方法建立骨質(zhì)疏松動(dòng)物模型。大鼠飼養(yǎng)3周后，分別以伊班膦酸鈉、骨髓間充質(zhì)干細(xì)胞、伊班膦酸鈉聯(lián)合骨髓間充質(zhì)干細(xì)胞對(duì)骨質(zhì)疏松動(dòng)物進(jìn)行干預(yù)治療，于給藥7周后檢測(cè)各組大鼠血清骨特異性堿性磷酸酶( bone alkaline phosphotase，BALP)、抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase-5b ，TRACP-5b)，并取大鼠股骨進(jìn)行壓縮、彎曲力學(xué)性能實(shí)驗(yàn)，檢測(cè)脛骨骨密度(bone mineral density，BMD)。結(jié)果 以伊班膦酸鈉聯(lián)合骨髓間充質(zhì)干細(xì)胞干預(yù)組脛骨 BMD、血清BALP高于模型組、以伊班膦酸鈉干預(yù)組、以骨髓間充質(zhì)干細(xì)胞干預(yù)組，其血清TRACP -5b 小于骨質(zhì)疏松動(dòng)物模型組、以伊班膦酸鈉干預(yù)組、以骨髓間充質(zhì)干細(xì)胞干預(yù)組；股骨各項(xiàng)壓縮和彎曲力學(xué)性能大于模型組、以伊班膦酸鈉干預(yù)組、以骨髓間充質(zhì)干細(xì)胞干預(yù)組，差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。結(jié)論 通過以伊班膦酸鈉聯(lián)合骨髓間充質(zhì)干細(xì)胞干預(yù)治療可使骨質(zhì)疏松動(dòng)物脛骨BMD、血清ALP升高、TRACP-5b水平下降，股骨的壓縮、彎曲強(qiáng)度和韌性可以得到一定恢復(fù)。 |
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Objective This paper verifies the effect of bone marrow mesenchymal stem cells (BMSCs) combined with ibandronate intervention on the recovery of mechanical properties such as compression and bending of bone after treatment of osteoporotic animals.Methods Sixty 5-month-old female SD rats were randomly divided into normal control group (n = 12), osteoporosis animal model group (n = 12), osteoporosis animal model with ibandronate intervention group (n = 12), osteoporosis animal model with BMSCs intervention group (n = 12), osteoporosis animal model with ibandronate combined with BMSCs intervention group (n = 12). The animal model of osteoporosis was established by ovariectomy. After 3 weeks of feeding, the rats were intervened with ibandronate sodium, BMSCs, ibandronate sodium combined with BMSCs. After 7 weeks of administration, the serum levels of bone alkaline phosphatase (BAL), tartrate-resistant acid phosphatase 5b (TRACP-5b) were detected, the femurs of rats were taken for compression and bending tests, and the bone mineral density of tibia (BMD) was also measured. Results The BMD of tibia and serum BALP in ibandronate sodium combined with BMSCs intervention group were higher than those in model group, ibandronate sodium intervention group and BMSCs intervention group,while the level of serum TRACP-5b in ibandronate combined with BMSCs intervention group was lower than that in osteoporosis animal model group, ibandronate group and BMSCs intervention group; Compression and bending mechanical properties of femur in ibandronate sodium combined with BMSCs intervention group were greater than those in model group, ibandronate sodium intervention group and BMSCs intervention group,the difference was significant (P < 0.05). Conclusion: Osteoporosis animals can recover with higher level of the BMD and serum BALP, lower level of TRACP-5b by using bisphosphonate combined with BMSCs stem cells, the femoral compression and bending strength and toughness were also greater.
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