北京生物醫(yī)學(xué)工程

離子溫度雙敏感型黏膜創(chuàng)面保護(hù)膠性能及黏膜修復(fù)有效性體外評(píng)價(jià)研究

Characterization of ion-temperature sensitive mucosal protective gel and effectiveness evaluation of mucosal repair in vitro

作者：楊敏一王涵曾行孟珠王春仁

單位：中國食品藥品檢定研究院（北京 102629），<br />中國藥科大學(xué)（南京 210009），<br />通信作者：王春仁。E-mail: [email protected]

關(guān)鍵詞：離子溫度雙敏感型黏膜創(chuàng)面保護(hù)膠凝膠；凝膠溫度；溶脹度；有效性；黏膜修復(fù)

分類號(hào)：R318.08 

出版年·卷·期（頁碼）：2022·41·4（405-412）

摘要：

目的為了開發(fā)一種內(nèi)鏡下黏膜切除術(shù)（endoscopic submucosal dissection，ESD）術(shù)后保護(hù)創(chuàng)面、修復(fù)黏膜損傷的生物材料，對(duì)離子溫度雙敏感型黏膜創(chuàng)面保護(hù)膠化性能和生物學(xué)性能進(jìn)行研究，評(píng)價(jià)其在ESD術(shù)后修復(fù)方面的可行性。方法采用倒瓶法、電子顯微鏡掃描、流變儀觀察樣品成膠特性、結(jié)構(gòu)表征，并通過重量法對(duì)凝膠溶脹度進(jìn)行評(píng)價(jià)，隨后進(jìn)行細(xì)胞增殖和細(xì)胞遷移實(shí)驗(yàn)，通過檢測(cè)細(xì)胞存活率、細(xì)胞遷移率評(píng)價(jià)凝膠有效性。結(jié)果離子溫度雙敏感型黏膜保護(hù)膠是一種致密的無孔隙結(jié)構(gòu)，在室溫下呈液態(tài)，在37℃，3min迅速成膠，成膠溫度在35℃左右。凝膠的最大溶脹度為238%。凝膠在浸提24h時(shí)浸提液能夠促進(jìn)人正常結(jié)腸上皮細(xì)胞( human normal colon epithelial cells,CCD 841 CoN cells)增殖，促進(jìn)增殖效果最佳濃度為50%，促進(jìn)遷移效果具有濃度依賴性，浸提液濃度為12.5%時(shí)，培養(yǎng)36h細(xì)胞遷移率達(dá)到67.5%，而陰性對(duì)照遷移率僅為27.1%，具有統(tǒng)計(jì)學(xué)差異（P<0.001）。結(jié)論離子溫度雙敏感型黏膜保護(hù)膠在ESD術(shù)后修復(fù)方面有一定的作用，對(duì)其在ESD術(shù)后并發(fā)癥的修復(fù)方面提供一定的參考。

Objective To develop a biomaterial to protect the lesion and promote wound healing in ESD. To study the physicochemical and biological properties of ionic-temperature sensitive mucosal protection gel and to evaluate the feasibility in ESD repair. Methods Using bottle filling method, electron microscope scanning and rheometer to study the gelatinization and structure characterization of the gel. The swelling degree of the gel was evaluated by gravimetric method. Cell proliferation and cell migration experiments indicated that the gel’s effectiveness. Results the ion-temperature sensitive mucosal protective gel was compact porous structure. It was liquid at room temperature and formed rapidly at 37℃ in 3min. The gel time was 35℃. The gel’s maximum swelling was 238%. When the gel was extracted for 24h, the extract could promote the proliferation of CCD 841 CoN cells, the optimal concentration was 50%. The effect of promoting migration was concentration-dependent. When the concentration of the extract was 12.5%, the mobility of cells reached was 67.5% for 36 h, while that of the negative control was only 27.1%, which has a statistical difference (P<0.001). Conclusions Ionic-temperature sensitive mucosal protective gel play a certain roal in the repair of postoperative ESD, which provides a certain reference for the repair of postoperative complications in ESD.

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