北京生物醫(yī)學(xué)工程

慢性高眼壓作用下視網(wǎng)膜功能及篩板形態(tài)改變

Changes of retinal function and morphology of lamina cribrosa under chronic ocular hypertension

作者：張景茜任璐頔錢秀清

單位：首都醫(yī)科大學(xué)生物醫(yī)學(xué)工程學(xué)院（北京 100069）<br />首都醫(yī)科大學(xué)臨床生物力學(xué)應(yīng)用基礎(chǔ)研究北京市重點(diǎn)實驗室（北京 100069）<br />通信作者：錢秀清。E-mail: [email protected]

關(guān)鍵詞：慢性高眼壓；視神經(jīng)；視覺電生理；篩板；孔隙率

分類號：R318.04

出版年·卷·期（頁碼）：2022·41·5（447-453）

摘要：

目的本研究通過視覺電生理測試技術(shù)和激光共聚焦圖像獲取技術(shù)研究慢性高眼壓作用下視網(wǎng)膜功能及篩板形態(tài)的改變情況，為青光眼致病機(jī)制研究提供基礎(chǔ)。方法通過建立慢性高眼壓動物模型，對正常大鼠和造模后2周、4周大鼠進(jìn)行閃光視網(wǎng)膜電圖（flash electroretinogram，F(xiàn)-ERG）、閃光視覺誘發(fā)電位（flash visual evoked potential，F(xiàn)-VEP）測試，并通過共聚焦顯微鏡對篩板組織切片進(jìn)行觀察，計算孔隙率。結(jié)果慢性高眼壓造模2周后，F(xiàn)-VEP的P1潛時下降、N2潛時顯著增長；造模4周后，F(xiàn)-ERG中Rod-ERG、Cone-ERG的b 波幅值顯著下降，Max-ERG的a波幅值和b波幅值均顯著下降，F(xiàn)lick-ERG的峰值幅值顯著下降，各反應(yīng)波形的潛時沒有明顯變化，鼠眼篩板孔隙率升高。結(jié)論慢性高眼壓作用2周后，視神經(jīng)的軸突、髓鞘功能下降。高眼壓作用4周后視網(wǎng)膜感光功能進(jìn)一步衰減，同時篩板組織膠原排列發(fā)生變化，篩孔面積比例增加。

Objective This study aims to study the changes of retinal function and morphology of lamina cribrosa under chronic ocular hypertension by using visual electrophysiological test and confocal microscope test, and to provide a basis for the pathogenic mechanism research of glaucoma. Methods By inducing a rat model of chronic ocular hypertension, we performed flash electroretinogram (F-ERG) and flash visual evoked potential (F-VEP) tests on normal rats and two weeks, four weeks rats after modeling, and observed the lamina cribrosa tissue slices through a confocal microscope, and then calculated the porosity of lamina cribrosa. Results Two weeks after chronic ocular hypertension, the results of P1 latency of F-VEP decreased and the N2 latency increased significantly compared with the control group. Four weeks after chronic ocular hypertension, the b-wave amplitude of Rod-ERG and Cone-ERG in F-ERG decreased, and the a-wave amplitude and b-wave amplitude of Max-ERG decreased, and the peak amplitude of Flick-ERG decreased significantly. There was no obvious change in the latency of each response waveform. The porosity of lamina cribrosa increased. Conclusions The function of optic nerve axon and myelin sheath of rats degrade after two weeks of chronic ocular hypertension. Four weeks after chronic ocular hypertension, the photoreceptive function of the retina degrades. While the arrangement of lamina beam changes and the proportion of lamina pores increases.

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