北京生物醫(yī)學(xué)工程

氧化鐵納米顆粒對(duì)靜止?fàn)顟B(tài)白血病細(xì)胞的作用研究

The effects of iron oxide nanoparticles on quiescent leukemia cells

作者：李宛清張雪溫濤劉健孟潔許海燕

單位：中國(guó)醫(yī)學(xué)科學(xué)院基礎(chǔ)醫(yī)學(xué)研究所，北京協(xié)和醫(yī)學(xué)院基礎(chǔ)學(xué)院（北京  100005）<br />通信作者：許海燕，E-mail：[email protected]；孟潔，E-mail：[email protected]

關(guān)鍵詞：氧化鐵納米顆粒；白血病細(xì)胞；靜止；氧化應(yīng)激；凋亡

分類(lèi)號(hào)： R318.04

出版年·卷·期（頁(yè)碼）：2023·42·1（52-56）

摘要：

目的研究二巰基丁二酸修飾的四氧化三鐵納米顆粒（DMSA-Fe3O4 NPs）對(duì)靜止?fàn)顟B(tài)白血病細(xì)胞的作用及機(jī)制。方法利用透射電鏡觀察納米顆粒形貌；用低血清培養(yǎng)基（0.5% FBS）誘導(dǎo)細(xì)胞進(jìn)入靜止?fàn)顟B(tài)，采用普魯士藍(lán)染色法與鄰二氮菲鐵定量法檢測(cè)DMSA-Fe3O4 NPs進(jìn)入Kasumi-1細(xì)胞的情況；通過(guò)乳酸脫氫酶法、細(xì)胞氧化應(yīng)激熒光探針和凋亡檢測(cè)試劑分析 DMSA-Fe3O4 NPs對(duì)Kasumi-1細(xì)胞的作用。結(jié)果 DMSA-Fe3O4 NPs可進(jìn)入靜止?fàn)顟B(tài)的Kasumi-1細(xì)胞，細(xì)胞內(nèi)鐵含量與DMSA-Fe3O4 NPs濃度成正比；與此同時(shí)，細(xì)胞活性降低，細(xì)胞內(nèi)ROS與脂質(zhì)過(guò)氧化物水平升高，細(xì)胞發(fā)生凋亡。正常培養(yǎng)的Kasumi-1細(xì)胞活性和氧化應(yīng)激水平不受DMSA-Fe3O4 NPs的影響。結(jié)論 DMSA-Fe3O4 NPs可進(jìn)入靜止?fàn)顟B(tài)的白血病細(xì)胞并引起細(xì)胞凋亡。

Objective To investigate the effect of dimercaptosuccinic acid modified ferric oxide nanoparticles (DMSA-Fe3O4 NPs) on quiescent acute myeloid leukemia cell line (Kasumi-1). Methods The morphology of DMSA-Fe3O4 NPs was observed by transmission electron microscopy. The low serum containing medium (0.5% FBS) was applied to induce Kasumi-1 cells to the quiescent state. The cellular uptake of DMSA-Fe3O4 NPs was analyzed by Prussian blue staining and phenanthroline spectrophotometric analysis. The release of lactate dehydrogenase, reactive oxidative species and apoptosis of Kasumi-1 treated with DMSA-Fe3O4 NPs at different concentrations were examined. Results The DMSA-Fe3O4 NPs entered the quiescent Kasumi-1 cells and the content of intracellular iron increased in a nanoparticles dose dependent manner. The viability of the quiescent Kasumi-1 cells was significant decreased and the intracellular ROS and lipid peroxide were increased as well. The Kasumi-1 cells cultured with the complete culture medium (10% FBS) was not affected by DMSA-Fe3O4 NPs. Conclusions DMSA-Fe3O4 NPs could preferably enter the quiescent Kasumi-1 cells, which resulted in the upregulation of intracellular oxidative stress and cell apoptosis.

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