北京生物醫(yī)學(xué)工程

心肌細胞電脈沖作用下電場分布仿真結(jié)果與分析

Simulation results and analysis of electric field distribution under the action of electrical pulses in cardiac muscle cells

作者：朱宇成王群山莫斌峰牛金海

單位：上海交通大學(xué) 生物醫(yī)學(xué)工程學(xué)院(上海  200240)<br />上海交通大學(xué)醫(yī)學(xué)院附屬新華醫(yī)院(上海  200092)<br />通信作者：牛金海，E-mail：[email protected]；莫斌峰，E-mail：[email protected] 

關(guān)鍵詞：心臟消融；心肌細胞；脈沖電場；電勢分布；仿真分析

分類號：R318.04

出版年·卷·期（頁碼）：2023·42·1（67-73）

摘要：

目的細胞電脈沖刺激仿真是研究心臟電脈沖消融的一種仿真方式，本文建立橢球形細胞電脈沖刺激仿真模型，模擬心肌細胞受到電脈沖刺激下的情況，研究電場入射方向和細胞長度對其電場分布和跨膜電位的影響。方法通過COMSOL5.5軟件進行仿真，以球形細胞模型為基礎(chǔ)，在邊長60μm的立方體空間中建立橢球形細胞模型。于垂直于Z軸的兩面施加2.4 V電壓，用以模擬心肌細胞在外加勻強電場作用下的電勢分布情況。改變脈沖電場與細胞長軸的夾角，研究0、30°、60°、90°的不同電場入射角度對心肌細胞電勢分布和跨膜電位的影響。保持入射角為0，研究跨膜電位最大值與細胞長軸直徑的關(guān)系。結(jié)果對于橢球形的心肌細胞，電場的入射角從0°增大到90°時，跨膜電位從1.068V減小至0.373V，同時最大跨膜電位的位置也發(fā)生改變。入射角為0°時，跨膜電位最大值V與細胞長軸直徑D的線性回歸方程為V_2=81.1916+38.6079D_2 ，r^2=0.9981。結(jié)論電場入射角越大，細胞跨膜電位越低；細胞長軸直徑越長，跨膜電位最大值越大。該研究對后續(xù)心肌細胞電脈沖刺激實驗及心臟電脈沖消融的臨床試驗具有參考意義。

Objective Cell electrical pulse stimulation simulation is a method to study cardiac electrical pulse ablation. In this paper, an ellipsoidal cell electrical pulse stimulation simulation model is established to simulate the situation of myocardial cells stimulated by electrical pulses, and to study the electric field distribution direction and cell length on the electric field and transmembrane potential. Methods Using COMSOL5.5 software for simulation, based on the spherical cell model, an ellipsoidal cell model is established to simulate the potential distribution of cardiomyocytes under the action of a uniform electric field. Apply 2.4 V voltage on both sides perpendicular to the Z axis to simulate the potential distribution of myocardial cells under the action of an external uniform electric field. By changing the angle between the pulsed electric field and the long axis of the cell, the effects of different electric field incidence angles of 0 °, 30 °, 60 ° and 90 ° on the potential distribution and transmembrane potential of the myocardial cell are studied. The relationship between the maximum transmembrane potential and the diameter of the long axis of cells is studied by keeping the angle of incidence at 0.Results For ellipsoidal cardiomyocytes, when the incidence angle of the electric field increases from 0 ° to 90 °, the transmembrane potential decreases from 1.068V to 0.373V, and the position of the maximum transmembrane potential also changes. When the incidence angle of the electric field is 0 °, the linear regression equation between the maximum transmembrane potential V and the cell long axis diameter D is V_2=81.1916+38.6079D_2 ，r^2=0.9981. Conclusions The greater the incident angle of electric field, the lower the transmembrane potential of cells; The longer the cell long axis diameter is, the greater the transmembrane potential is. This study has reference significance for the subsequent experiment of myocardial cell electric pulse stimulation and the clinical trial of cardiac electric pulse ablation.

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