北京生物醫(yī)學(xué)工程

基于光纖的熒光成像及熒光強(qiáng)度測量系統(tǒng)

An Optical Fiber-Based Fluorescence Imaging and Fluorescence Intensity Measurement System

作者：陳頎瀟白凈

單位：清華大學(xué)生物醫(yī)學(xué)工程系（北京100084）

分類號：

出版年·卷·期（頁碼）：2010·29·5（470-474）

摘要：

基于光纖的熒光成像及熒光強(qiáng)度測量系統(tǒng)由作為激發(fā)光源的發(fā)光二極管、作為熒光探測器的電荷耦合器件、光纖探頭以及用于系統(tǒng)控制和數(shù)據(jù)處理的計(jì)算機(jī)軟件構(gòu)成。當(dāng)光纖探頭使用傳像光纖時，本系統(tǒng)實(shí)現(xiàn)顯微熒光成像功能，可以對使用熒光標(biāo)記的細(xì)胞進(jìn)行離體或者在體觀察；當(dāng)光纖探頭使用“1分4”傳光光纖時，系統(tǒng)可實(shí)現(xiàn)多通道實(shí)時熒光強(qiáng)度測量功能，且能夠以微創(chuàng)的方式對實(shí)驗(yàn)動物體內(nèi)多個器官進(jìn)行在體、實(shí)時及連續(xù)的熒光強(qiáng)度測量，為研究熒光染料在各器官內(nèi)的代謝情況提供了一種新的工具。

The optical fiber-based fluorescence imaging and intensity measurement system contains the LED excitation light source, the CCD, the optical fiber probe, and the software which is used to control the system and process the data. When the image fiber is used as the optical fiber probe, a new in-vivo and in-vitro microscopic fluorescence imaging application can be achieved to observe the fluorescence marked cells. When the special “4-in-1” light fiber is used as the optical fiber probe, the system provides an in-vivo, multi-channel, real-time, and continuous fluorescence intensity measurement application, and it facilitates the study of the metabolism of the fluorescence dye in the animal organs.

參考文獻(xiàn)：

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