北京生物醫(yī)學(xué)工程

基于連筆直寫的微結(jié)構(gòu)血管支架體外測(cè)評(píng)

In vitro Evaluation of the Microstructural Vascular Scaffold with MicroTip Direct Writing Technology

作者：孫平平胡川周建業(yè) 吳任東沈雅胡盛壽

單位：中國醫(yī)學(xué)科學(xué)院阜外心血管病醫(yī)院，衛(wèi)生部心血管再生醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室 (北京100037)

分類號(hào)：

出版年·卷·期（頁碼）：2011·30·2（111-116）

摘要：

目的評(píng)價(jià)基于連筆直寫的生命體微結(jié)構(gòu)成形技術(shù)制備的管狀支架的力學(xué)性能及其生物相容性。方法對(duì)該血管支架采用徑向順應(yīng)性、縫合強(qiáng)度、爆破壓力等力學(xué)性能檢測(cè)；通過溶血率、體外動(dòng)態(tài)凝血實(shí)驗(yàn)、血小板黏附實(shí)驗(yàn)進(jìn)行血液相容性的分析；采用細(xì)胞培養(yǎng)MTT法及細(xì)胞形態(tài)學(xué)觀察方法，研究其細(xì)胞相容性。結(jié)果血管支架的徑向順應(yīng)性為（4.03±0.56）%/100mmHg，縫合強(qiáng)度為（204.5±72.1）N/cm2，爆破壓力約為（102±8）kPa；支架的溶血率為1.75%，小于ISO規(guī)定的5%；在體外動(dòng)態(tài)凝血實(shí)驗(yàn)中，血管支架的抗凝血性能顯著優(yōu)于對(duì)照組載玻片（P＜0.05），而且掃描電鏡觀察到血小板黏附較少，顯示出該血管支架具有良好抗凝血性能；MTT比色法結(jié)果顯示其細(xì)胞毒性為0～1級(jí)；細(xì)胞形態(tài)學(xué)觀察顯示L929細(xì)胞在該血管支架膜片的浸提液中呈梭形或三角形，貼壁良好。結(jié)論該血管支架具有良好的力學(xué)性能、血液相容性和細(xì)胞相容性，可以滿足組織工程血管支架的要求。

Objective To evaluate the mechanical properties and biocompatibilities of the vascular scaffold manufactured with micro-tip direct writing technology.Methods Mechanical properties of the vascular scaffold were measured by the parameters of radial compliance，suture strength and bursting pressure separately.The blood compatibilities of the scaffold were analyzed by hemolytic rate test，in vitro dynamic blood clotting test，ant platelet adhesion experiment.Cell compatibility was evaluated by MTT measurement and morphological observation.Results The scaffold showed radial compliance of (4.03±0.56)%/100mmHg，burst strength of (102±8)kPa，and suture retention strength of (204.5±72.1)N/cm2.The hemolytic rate was 1.75%，being lower than 5％，which was ruled by ISO.The anti-coagulant property of the scaffolds was significantly better than that of glass slides in dynamic coagulation tests（P＜0.05).The anti-coagulant property of the scaffold was also confirmed by less platelet adhesion.MTT assay results showed that the cytotoxic grade of the novel polyurethane was from 0 to 1.Cell morphology observation showed that the L929 cells were spindle-shaped or triangular with good stretch.Conclusion The scaffold manufactured with the new technique processes good mechanical properties，blood compatibility and cell compatibility.

參考文獻(xiàn)：

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