北京生物醫(yī)學工程

銀納米顆粒對神經(jīng)元的毒性研究

Toxicity of Silver Nanoparticles to the Neurons

作者：周國鳳湯京龍熊玲周亮許建霞邵安良楊麗娜陳艷梅李雪飛奚廷斐

單位：溫州醫(yī)學院（浙江溫州325035）

關(guān)鍵詞：銀納米顆粒；神經(jīng)元；毒性

分類號：

出版年·卷·期（頁碼）：2011·30·3（301-305）

摘要：

目的研究不同濃度的銀納米顆粒對神經(jīng)元毒性的劑量-效應(yīng)關(guān)系，探索銀納米顆粒對神經(jīng)元的毒性機理。方法首先培養(yǎng)一種活性好、生長狀態(tài)優(yōu)良的原代神經(jīng)元，將不同濃度（2.5~500μg/mL）的銀納米顆粒加入神經(jīng)元中作用24h后，通過MTT法計算細胞增殖率，并分析不同濃度的銀納米顆粒對神經(jīng)元毒性的劑量-效應(yīng)關(guān)系。結(jié)果在25~250μg/mL范圍內(nèi)，細胞數(shù)量與形態(tài)呈現(xiàn)不同的變化，銀納米顆粒濃度與細胞增殖率呈負相關(guān)。結(jié)論在25~250μg/mL的濃度范圍內(nèi)，銀納米顆粒對神經(jīng)元細胞的毒性呈劑量-效應(yīng)關(guān)系。

Objective To study the dose-effect relationship between silver nanoparticles with different concentrations and the neurotoxicity, and also explore the mechanism of silver nanoparticles to neurotoxicity. Method A group of primary neurons in excellent growth status was cultured, and then contacted with different concentrations (2.5-500μg/ml )of silver nanoparticles for 24h. After that we calculated the cell proliferation rate by MTT test and analyzed the dose-effect relationship between the concentrations of silver nanoparticle with neurotoxicity. Result Within the silver nanoparticle concenrtation of 25-250μg/ml, both cellular mount and morphology changed, and it was showed anticorrelation between silver nanoparticle concentration and cell proliferation rate. Conclusion Within the sliver nanoparticles concenrtation of 25-250μg/ml, silver nanoparticles shows a dose-effect relationship on neurotoxicity.

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