北京生物醫(yī)學(xué)工程

基于微流控芯片的姜黃素誘導(dǎo)MCF-7細胞凋亡的研究

Microfluidic Chip-based Apoptosis of MCF-7 Induced by Curcumin

作者：王雪莉錢翔謝振華葉大田

單位：清華大學(xué)生物醫(yī)學(xué)工程系(北京 100084)

分類號：

出版年·卷·期（頁碼）：2011·30·6（557-561）

摘要：

目的探討應(yīng)用微流控芯片實現(xiàn)高內(nèi)涵藥物篩選(high content screening, HCS)的可行性。方法本文將微流控芯片技術(shù)與HCS技術(shù)相結(jié)合，通過自行設(shè)計、制作聚二甲基硅氧烷(polydimethylsiloxane, PDMS)-玻璃微流控芯片，并在芯片上實現(xiàn)人乳腺癌MCF-7細胞培養(yǎng)、脂質(zhì)體轉(zhuǎn)染、藥物姜黃素刺激等操作，最后通過顯微成像技術(shù)進行檢測。結(jié)果姜黃素可以誘導(dǎo)MCF-7細胞凋亡，并呈濃度依賴性，同時獲得了細胞在凋亡過程中一些生物信息的改變：隨著姜黃素濃度的增加，細胞凋亡比例、Endo G-GFP重定位比例增大，膜通透性增加，細胞核固縮變小。結(jié)論上述微流控芯片可以為HCS技術(shù)提供良好的研究平臺。

Objective To discuss the feasibility of microfluidic chip to realize high content screening (HCS). Methods In this paper, microfluidic chip and HCS were successfully combined together. We fabricated a microfluidic chip made from polydimethylsiloxane(PDMS) and glass, then accomplished the culture of MCF-7 cell line, the transfection of Endo G-GFP, and the stimulation of curcumin on this chip, finally acquired data with a microscopic imaging system. Results Curcumin could induce the apoptosis of MCF-7, and the effect relied on the concentration. The information related to apoptosis were obtained: with the increasing of the concentration of curcumin, the percentage of apoptotic cells and the proportion of relocation of Endo G-GFP went up, the permeability of plasma membrane increased, and the nuclear size diminished. Conclusions All the results demonstrated that microfluidic chip could provide a good platform for HCS.

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