北京生物醫(yī)學(xué)工程

松質(zhì)骨移植材料孔隙率的顯微CT和組織切片測試方法比較

The cancellous bone graft substitute porosity tested by the micro CT image method and the biopsy image method

作者：曹帥趙改平顏文濤

單位：上海理工大學(xué)生物力學(xué)與康復(fù)工程研究所(上海200093)

分類號：

出版年·卷·期（頁碼）：2012·31·5（478-481）

摘要：

目的采用顯微CT法和組織切片法對松質(zhì)骨移植材料的孔隙率進(jìn)行對比研究，為骨組織工程支架材料的研制和開發(fā)提供理論依據(jù)。方法首先取新鮮尸體的股骨和新鮮豬股骨，進(jìn)行去除多余組織、滅菌、低溫冷凍保存等處理后，制備成松質(zhì)骨移植材料，使用顯微CT分別掃描同種異體骨和動物源性豬骨移植材料獲取顯微CT圖像。然后對同種異體松質(zhì)骨組織進(jìn)行染色和切片等處理，制備成同種異體骨組織切片材料，顯微鏡下觀察骨組織切片并獲取其圖像，各取10幅圖像用于實驗。設(shè)計matlab程序?qū)λ袌D像進(jìn)行灰度化、圖像增強、二值化、移除干擾等處理，計算孔隙率的數(shù)值。結(jié)果顯微CT和組織切片測試得到的同種異體松質(zhì)骨孔隙率與動物源性豬松質(zhì)骨孔隙率結(jié)果無顯著性差異(P>0.05)。結(jié)論顯微CT標(biāo)本的制備較組織切片大為簡化，可以無損地獲取數(shù)據(jù)，并省去灰度化、圖像增強、移除干擾等處理過程，比組織切片法測量松質(zhì)骨孔隙率更加簡便。

Objective To compare the cancellous bone graft substitute porosity by micro CT image method and biopsy image method, to provide a theoretical basis for scaffold material research and development of bone tissue engineering. Methods First, the femur of a fresh human body and a fresh pig body were taken to remove excess tissue, for sterilization, cryopreservation and other treatments to prepare for cancellous bone graft material, and to obtain the images of allograft bone and animal pig bone graft materials under micro-CT scan. Then by staining and cutting allogeneic cancellous bone tissue, we prepared for bone tissue section material, observed allograft bone tissue sections under a microscope and got 10 images for each section in the three groups. Finally matlab program was designed for grayscale, enhancement, binarization and removing the interference to calculate the value of the porosity. Results The porosity of allogeneic bone and animal pig cancellous bone graft materials that were measured by micro CT method and tissue section imaging method showed no significant differences(P>0.05). Conclusions Compared with tissue section, the preparation of micro CT specimen are simplified, non-destructive and eliminating the processes for grayscale, image enhancement and interference cancellation. Therefore, the micro CT image method for the cancellous bone graft substitute porosity is more convenient than the biopsy image method.

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