北京生物醫(yī)學(xué)工程

海藻酸鈉復(fù)合物治療膝關(guān)節(jié)軟骨缺損的研究進(jìn)展

Sodium alginate complex used in the treatment of cartilage defect

作者：張伊韋登明逯卓卉蔣雯雯尹向旭陳偉

單位：寧波大學(xué)(浙江寧波315211)

關(guān)鍵詞：海藻酸鈉；組織工程；膝關(guān)節(jié)；軟骨；缺損

分類號：

出版年·卷·期（頁碼）：2012·31·6（649-651）

摘要：

膝關(guān)節(jié)是人體主要承重關(guān)節(jié)，其軟骨缺損是組織工程技術(shù)中正著力解決的難題。軟骨組織中細(xì)胞含量
少，主要成分是膠原蛋白和蛋白聚糖，修復(fù)能力有限。海藻酸鈉因其特殊的固液型可轉(zhuǎn)換特性、良好的生
物相容性，以及與軟骨基質(zhì)類似的結(jié)構(gòu)，而成為修復(fù)軟骨缺損的重要支架材料。很多實(shí)驗(yàn)支持海藻酸鈉復(fù)
合物支架治療膝關(guān)節(jié)軟骨缺損。三維培養(yǎng)是軟骨組織工程細(xì)胞體外培養(yǎng)的重要環(huán)境條件。自體軟骨細(xì)胞、
分化前或未分化的骨髓間充質(zhì)干細(xì)胞和基因轉(zhuǎn)染的細(xì)胞等均可獲得良好的治療效果。前期研究結(jié)果發(fā)現(xiàn)海
藻酸鈉通過動態(tài)壓縮加載、流體混合優(yōu)化、微流體等有極好的培養(yǎng)效果。在軟骨細(xì)胞的體外培養(yǎng)或誘導(dǎo)培
養(yǎng)中，要立足于為細(xì)胞創(chuàng)造與體內(nèi)類似的環(huán)境。海藻酸鈉復(fù)合物支架趨于復(fù)合化、復(fù)雜化，應(yīng)用上要具體
分析支架的孔隙率、力學(xué)強(qiáng)度、生物相容性等各方面數(shù)據(jù)。

Knee is the main bearing joint，whose cartilage defect is the important problem
in tissue engineering techniques. Cartilage is composed of collagen protein and proteoglycan
and a few cells. So the repair ability of cartilage is limited. Sodium alginate can transfer
between solid and liquid，has good biocompatibility and is similar with cartilage matrix.
Sodium alginate is the important scaffold material in tissue engineering. Many studies show
sodium alginate complex can be used in the treatment of knee-joint cartilage defect. 3D
environment culture is important in cell culture in vitro. Autologous chondrocytes，pre-
differentiated and undifferentiated mesenchymal stem cells and gene-transferred cells and so
forth are the ideal seed cells. Dynamic compressive loading，media mixing and microfluidic
technology achieve good culture effect. We should create environment similar to the original
for the cells. Scaffolds of sodium alginate complex tend to be complicate. We need to
analyze the data of porosity，mechanical strength，biocompatibility and etc.

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