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Objective To investigate the potential of adipose derived stem cells in reconstruction, we cultured and induced the adipose mesenchymal stem cells which were obtained from porcine adipose tissue, then set up a model of breast reconstruction with the skin expander in minipig’s subcutaneous abdominal adipose tissue. Methods Porcine adipose derived stem cells were obtained from subcutaneous adipose tissue of minipig with collagenase digestion, identified by flow cytometric analysis and induced to adopogenic and osteogenic differentiation in vitro. Results The stem cells were negative for CD34 and CD45, positive for CD44 and CD105. The cells were positive for oil red O staining and alizarin red staining. The local cells were much more in experimental group than in control group. Conclusions Porcine adipose derived stem cells can be cultured in vitro and induced adopogenic and osteogenic differentiation. The cells can be used in breast reconstruction model in vivo.