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體外動物組織熱變過程中的光學(xué)特性變化 In vitro experimental study on changes in the optical properties of animal tissues in the thermal denaturation |
| 作者： 張祿鵬 孫智 劉筑聞 李文志 張志宏 黃劍鋒 段元民 董勇 梁志遠(yuǎn) |
| 單位： 首都醫(yī)科大學(xué)生物醫(yī)學(xué)工程學(xué)院（北京100069） |
| 關(guān)鍵詞： 動物組織；熱變性；光學(xué)特性；積分球 |
| 分類號： |
| 出版年·卷·期（頁碼）：2014·33·5（502-507） |
| 摘要： |
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目的 研究熱變過程中生物組織的光學(xué)特性變化。方法 采用犬肝、犬腎、犬肌肉和鼠肌肉4種動物組織作為樣本，使用積分球系統(tǒng)測量650nm波長激光照射下的樣本在熱風(fēng)槍從30℃升溫到100℃過程中的準(zhǔn)直透射光、漫透射光和漫反射光光強(qiáng)隨溫度變化的數(shù)據(jù)，根據(jù)Kubelka-Munk模型計算得出吸收系數(shù)、散射系數(shù)和散射各向異性因子變化曲線。結(jié)果 溫度變化使組織光學(xué)特性改變，熱變過程中樣本透射減弱，吸收系數(shù)和散射系數(shù)增大，散射各向異性因子減小，不同組織的變化有一定差別。結(jié)論 熱變性使組織透射減弱，吸收和散射增強(qiáng)。 |
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Objective To explore optical property changes of biological tissues during thermal denaturation. Methods Dog livers, dog kidneys, dog muscles and rat muscles were used as four kinds of samples in the experiment. The integrating-sphere system measured intensity changes of collimated transmission light, diffuse transmission light and reflection light, when samples were heated from 30℃ to 100℃ by a hot air gun at 650nm laser irradiation. The curves of absorption coefficient, scattering coefficient and scattering anisotropy factor were calculated with the Kubelka-Munk model. Results Optical properties of tissues were affected by temperature. The transmittance and scattering anisotropy factor of samples decreased, while absorption and scattering coefficients increased during thermal denaturation. Conclusions Thermal denaturation of tissues cause transmission weakened, absorption and scattering enhanced. |
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地址：北京安定門外安貞醫(yī)院內(nèi)北京生物醫(yī)學(xué)工程編輯部 電話：010-64456508 傳真：010-64456661 電子郵箱：[email protected] |