北京生物醫(yī)學工程

骨髓間充質干細胞-脫細胞真皮基質對大鼠表皮缺損的修復

Acellular dermal matrix with bone marrow mesenchymal stem cells for the repair of skin defect in SD rat

作者：盧麗先史東曄劉英奇孫瑩崔巍巍

單位：                                 邯鄲市中心醫(yī)院口腔科(河北邯鄲056000)            

關鍵詞：骨髓間充質干細胞；脫細胞真皮基質；修復；缺損

分類號：

出版年·卷·期（頁碼）：2015·34·2（185-188）

摘要：

目的研究骨髓間充質干細胞(bone marrow mesenchymal stem cell, BMSC)-脫細胞真皮基質(acellular dermal matrix, ADM) 對SD大鼠皮膚表皮缺損的修復。方法培養(yǎng)SD大鼠骨髓細胞，增殖提純傳代至4代時，成骨成脂，流式細胞儀檢測鑒定BMSC，然后將其接種到ADM，2d后移植到皮膚表皮缺損的SD大鼠體內，連續(xù)5周觀察其修復效果。結果 BMSC-ADM修復SD大鼠皮膚表皮缺損效果良好，5周后，肉眼可見材料與組織結合緊密，有彈性，顏色接近皮膚。結論 BMSC-ADM可以作為修復SD大鼠皮膚表皮缺損的組織工程材料。

Objective To investigate acellular dermal matrix (ADM) combined with bone marrow mesenchymal stem cells (BMSC) for the repair of skin defects in SD rat. Methods We identified BMSCs by flow cytometry after the bone marrow cells of SD rats were cultured, proliferated, purified, passaged, and then we inoculated BMSCs into ADM. After two days, the ADM combined with BMSC was transplanted into the skin defect of SD rats to observe the repairing effect for five weeks. Results The repairing effect of ADM combined with BMSC in the skin defect of SD rats was good. The visible material was linked closely and flexibly with the organization of SD rats, and the color was closed to the normal skin after five weeks. Conclusions ADM combined with BMSC could be used as a tissue engineering material for repairing the skin defect of SD rats.

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