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Objective To establish one method of the determination of arsenic and mercury in injectable sodium hyaluronate gel by atomic fluorescence spectrometry. Methods The mercury and arsenic contents in samples were determined by atomic fluorescence spectrometry after microwave digestion. Results The concentrations of arsenic and mercury kept good and wide linear relationships with fluorescence intensity, and the coefficient correlations were 0.9999% and 0.9997%, respectively. The detection limits of arsenic and mercury in this method were 0.0105ng/mL and 0.0032ng/mL, respectively. Precisions both showed RSD＜0.5%. The average adding standard recovery rates of arsenic and mercury were 95.2% and 100.2%, respectively. The content of arsenic was below the detection limit(＜0.49 ng/g) while the content of mercury was 7.22 ng/g. Conclusions This microwave digestion-atomic fluorescence spectrometry applied to determine the mercury and arsenic contents in injectable sodium hyaluronate gel had the advantages of high accuracy and precision, good linear relationship and repeatability.