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Objective In this study we used force sensor and BL-420S system to observe stretching induced intact detrusor strip myography changes on time phases and evaluate SOCS1 expression under varying stretching overload conditions in order to investigate the mechanism of SOCS1 and the urinary bladder physiological and pathological changes under overload conditions. Methods Intact detrusor strip were from urinary bladder wall of Kunming mice. Preparations were fixed on a micro positioning device at slack position. Primary stretching preparation to passive tension 1 gram,this length was determined as the initial length (L0). Intermittently step stretching preparation 10 times (from L0+1 to L0+10),we recorded and analyzed stretching induced myography. SDS-PAGE and western blotting methods analyzed the expressions of suppressor of cytokine signaling 1(SOCS1) in L0+5 and L0+10 stretched preparations. Results (1) Micro stepping stretch induced instantaneously passive tension (PT) changes and subsequent vibration (Vib) could be recorded by force sensor and recording system. Myography patterns are variously dependent on pre-treatment conditions. (2) SOCS1 expression was significantly increased in intensive stretching overload preparations. Conclusions Myography reliably responds to overload conditions,instantaneously passive tensions and subsequently adaptation changes in intact detrusor preparation. Detrusor overload induces significantly increasing of SOCS1,which reveals it’s importance in detrusor protective remodeling. This detrusor micro stepping stretch model in vitro is a helpful method for understanding urinary bladder physiological and pathological changes under overload conditions.