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Objective This paper describes two methods of biochip preparation by roller biochip microarrayer and the calculation method of production cycle.We analyze and compare the work efficiency of the two methods in order to provide theoretical basis for improving the preparation efficiency of biological chip.Methods The traditional static spotting method adopts a new S curve acceleration and deceleration control method to avoid the overshoot or the vibration caused by frequent start-stop.The continuous dynamic spotting method adopts the genetic algorithm to solve the optimal solutions of the roller’s speed and the spotting times in each lap.We calculate the production cycle of biochip by calculating the time in each stage and verify the calculation accuracy by the experiment.Results The production cycle of biochip by the traditional static spotting method is 1173 seconds,and the production cycle of biochip by the continuous dynamic spotting method is 846 seconds.The efficiency increases by 27.8%.Conclusions The continuous dynamic spotting method is much more effective,more efficient and more conform to the requirements of the industry than the traditional static spotting method.