北京生物醫(yī)學(xué)工程

新型生物可吸收房間隔缺損封堵器的生物相容性研究

Biocompatibility study of a new type of biodegradable septal defect occluder

作者：謝兆豐王樹水張智偉劉香東陳賢淼張貴張德元

單位： 廣東省心血管病研究所，廣東省華南結(jié)構(gòu)性心臟病重點(diǎn)實(shí)驗(yàn)室，廣東省醫(yī)學(xué)科學(xué)院，廣東省人民醫(yī)院  (廣州510100)

關(guān)鍵詞：聚左旋乳酸；房間隔缺損；封堵器；生物相容性；生物降解性；溶血率

分類號：R318.08

出版年·卷·期（頁碼）：2016·35·6（582-587）

摘要：

目的評價自主研究的新型房間隔封堵器的生物相容性，為動物實(shí)驗(yàn)提供依據(jù)。方法封堵器框架由生物可吸收聚左旋乳酸（poly-L-lactic acid, PLLA）單絲網(wǎng)編織成型。通過對PLLA單絲進(jìn)行體外細(xì)胞毒性測試、體外溶血測試、全身急性毒性測試、肌肉植入實(shí)驗(yàn)，評價新型房間隔封堵器的生物相容性。結(jié)果 PLLA房間隔缺損封堵器無體外細(xì)胞毒性，體外溶血率0.9%，無急性全身毒性反應(yīng)。通過分析樣品植入肌肉1個月、3個月和6個月后組織病理，觀察樣品與組織之間的反應(yīng)，未發(fā)現(xiàn)明顯的組織損傷和組織增生。結(jié)論生物可吸收PLLA房間隔封堵器的生物相容性良好，可進(jìn)行下一步的動物實(shí)驗(yàn)研究。

Objective To design an atrial septal defect (ASD) occluder made of biodegradable poly-L-lactic acid (PLLA) and to evaluate its biocompatibility in vitro. Methods Bio-absorbable PLLA monofilament was used as occluder frame. Cytotoxicity test, acute toxicity test, hemolysis test and muscle implantation of PLLA monofilament were selected to evaluate the biocompatibility of the occluder. Results The PLLA monofilament had no cytotoxicity, no acute systemic toxicity and its hemolysis rate was 0.9% in vitro. No obvious tissue damage and tissue proliferation were observed at 1, 3 and 6 months after implantation of the samples. Conclusions The occluder made of bioabsorbable PLLA has good biocompatibility and can be selected for the next animal experimental study.

參考文獻(xiàn)：

［1］戴柯,李奮,孫康,等. 全生物可降解型房間隔缺損封堵器封堵豬房間隔缺損的實(shí)驗(yàn)研究［J］. 臨床兒科雜志, 2010, 28(4):375-379.

Dai Ke, Li Fen, Sun Kang, et al. Experimental ASD closure using a fully biodegradable ASD occluder in swine models［J］. J ClinPediatr, 2010, 28(4):375-379.

［2］Zhu YF, Huang XM, Cao J, et al. Animal experimental study of the fully biodegradable atrial septal defect (ASD) occlude［J］. J Biomed Biotechnol, 2012，（5）:735989.

［3］韓蓉, 劉彥斌, 張同成,等. 生物醫(yī)用材料的生物相容性評價［J］. 蘇州大學(xué)學(xué)報:醫(yī)學(xué)版, 2010, 30（4）: 773-776.

Han Rong, Liu Yanbin, Zhang Tongcheng, et al. Biocompatibility evaluation of biological materials［J］. Suzhou University Journal of Medical Science, 2010, 30（4）:773-776.

［4］朱玉峰, 王貴明, 黃新苗,等. 生物可吸收心臟間隔缺損封堵器的生物相容性研究［J］. 介入放射學(xué)雜志, 2013, 22(9):756-760.

Zhu Yufeng, Wang Guiming, Huang Xinmiao, et al. Experimental study of the biocompatibility of a self-made bio-absorbable occluder used for cardiac septal defect［J］. J Intervent Radiol, 2013, 22(9):756-760.

［5］Oberhauser JP, Hossainy S, Rapoza RJ. Design principles and performance of bioresorbable polymeric vascular scaffolds［J］. Euro Intervention, 2009, 5(Suppl F):F15-F22.

［6］Gupta B, Revagade N, Hilborn J. Poly(lactic acid) fiber: an overview. ［J］ Prog Polym Sci, 2007, 34:455-82.

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