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Backgrounds Hyperplasia of cervical uncinate joint usually exists in multi-level cervical segments，the surgical planning needs to consider a variety of factors because of the disease complexity. Clinicians find that two-level cervical segments’ unilateral microsurgical anterior cervical foraminotomy for radiculopathy after anterior decompression does not necessarily require interbody fusion. Objective To investigate biomechanical stability of cervical spine after two-level cervical segments’ unilateral microsurgical anterior cervical foraminotomy for radiculopathy. Methods Based on the CT scan images of an adult male volunteer，a three-dimensional geometric model of the cervical spine (C2-C7) was established by using Mimics13.1，SolidWorks2012 and ANSYS15.0 softwares. And then the mechanical parameters were assigned according to the material characteristic of tissues. Three-dimensional finite element model of the cervical spine was eventually created by using tetrahedral mesh method. The stability of cervical spine and the stress change of adjacent vertebral segment were analyzed by using the finite element analysis technique after two-level cervical segments’ unilateral microsurgical anterior cervical foraminotomy. Results The results showed that the maximum total deformation of each condition relative to the other three resections definitely increased after the removal of two-level cervical segment (60+60 model) by using the method described in this paper. Moreover，the various conditions of maximum equivalent stress significantly increased relative to the other three resections. Conclusions With the increase of the removed segments and the removal scope，the activity between the segments increases and the overall stability of the cervical spine are weakened. Increase in local cervical stress leads to cervical instability and accelerates the degeneration of the cervical spine. Therefore，we should strictly control surgical indications in surgical planning. The scope of resection cannot be blindly expanded unless there are certain clear indications，such as compression of nerve roots and spinal cord.